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Related Experiment Videos

[An improved assay for plasma tissue factor activity]

F Murakami1, K Iijima, C Fukuda

  • 1Department of Clinical Laboratory Medicine, Faculty of Medicine, Tottori University, Yonago.

Rinsho Byori. the Japanese Journal of Clinical Pathology
|February 1, 1995
PubMed
Summary
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This study presents an improved assay for measuring tissue factor activity in plasma. The new method is more sensitive and requires less sample preparation than previous techniques.

Area of Science:

  • Biochemistry
  • Hematology
  • Clinical Chemistry

Context:

  • Tissue factor (TF) plays a crucial role in the extrinsic pathway of coagulation.
  • Accurate measurement of plasma TF activity is essential for understanding hemostasis and thrombosis.
  • Existing assays for TF activity often require complex sample preparation, limiting their routine use.

Purpose:

  • To develop and validate a more sensitive and efficient assay for measuring tissue factor activity in plasma.
  • To compare the performance of the improved assay with a conventional method.
  • To establish reference values for plasma TF activity in healthy individuals.

Summary:

  • An improved assay utilizing chromogenic substrate S-2765, sensitive to factor Xa, was developed for plasma tissue factor (TF) activity measurement.

Related Experiment Videos

  • This assay requires only 20-fold diluted plasma and Owren's barbital buffer, eliminating the need for euglobulin fraction preparation and heating.
  • Absorbance at 405nm was measured using a colorimeter against human placental tissue thromboplastin (HPT) standards, yielding a mean value of 15.8 +/- 16.4 µg/ml HPT in normal subjects (mean +/- 2SD).
  • A correlation of 0.62 was observed between the improved assay and the ordinary method, indicating good agreement.

    • Impact:

    • The developed assay offers a more sensitive and streamlined approach for quantifying plasma TF activity.
    • This improved method has the potential to enhance diagnostic capabilities in thrombotic and hemostatic disorders.
    • Facilitates more accessible and reliable assessment of coagulation status in clinical settings.