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Detection of herpes simplex virus type 2 Bgl II N fragment in paraffin-embedded cervical tissue sections using nested
V Lulitanond1, W Chantratita, K Thammaprasert
1Department of Microbiology, Faculty of Science, Mahidol University, Bangkok, Thailand.
Molecular and Cellular Probes
|December 1, 1994
Abstract:
To study the presence of transforming sequence Bgl II N of HSV-2 in cervical cancer tissues, we developed the nested polymerase chain reaction (PCR) for detecting such a sequence in paraffin-embedded cervical tissue sections. Samples derived from 46 patients with premalignant and malignant lesions were tested. The sequence was found in 20-25% of total cases tested but not observed in any of the normal healthy controls. This study also indicates that for the detection of HSV-2 Bgl II N sequence in cervical tissue, the nested PCR may be more reliable than the in situ hybridization method.