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[The genetic polymorphism of cereals demonstrated by PCR with random primers]
Tsitologiia I Genetika
|November 1, 1994
Abstract:
Polymerase chain reaction of DNA amplification with the use of random primers is a new approach in investigations of genome specificity. Nucleotide sequences that showed polymorphism in RFLP analysis were used as primers. Optimal temperature conditions and Mg2+ concentrations were determined for studying inter- and intraspecific DNA polymorphism in the most important cereals.