[A synthetic medium containing growth factors for the cultivation of producer cell lines]

A synthetic medium with low serum contents has been composed. As basic substitutes for serum a mixture containing a fibroblast growth factor, insulin, albumin and fibronectin is proposed. It allows to carry out a long-term cultivation of RH-PA cells, which are producers of a plasminogen activator, both in static conditions and on microcarriers. The suitability of the suggested medium for cultivation of other biotechnologically important cell lines (SPEV, NIH 3T3, Vero, CHO) is demonstrated.