Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Expression of ectopeptidases in scleroderma

G Bou-Gharios1, J Osman, A Atherton

  • 1Cell Enzymology Unit, Kennedy Institute of Rheumatology, London, United Kingdom.

Annals of the Rheumatic Diseases
|February 1, 1995
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A multi-centre real-world evaluation of AI-assisted organ at risk contouring on radiotherapy treatment planning workflows.

The British journal of radiology·2026
Same author

Rising seroprevalence of Borrelia spp. in free roaming felines in the eastern Tennessee valley.

Veterinary parasitology·2025
Same author

Comparing physiological impacts of positive pressure ventilation versus self-breathing via a versatile cardiopulmonary model incorporating a novel alveoli opening mechanism.

Computers in biology and medicine·2024
Same author

Joint replacement risk is markedly increased in alkaptonuria (AKU) in those with prior arthroplasty.

Molecular genetics and metabolism reports·2024
Same author

Hepatobiliary circulation and dominant urinary excretion of homogentisic acid in a mouse model of alkaptonuria.

Journal of inherited metabolic disease·2024
Same author

Molecular architecture of the ciliary tip revealed by cryo-electron tomography.

bioRxiv : the preprint server for biology·2023

Scleroderma patients show reduced levels of dipeptidyl peptidase IV (DPPIV) on skin cells, potentially explaining increased adhesion molecules and matrix proteins in this fibrotic disease.

Area of Science:

  • Dermatology
  • Immunology
  • Biochemistry

Background:

  • Scleroderma (systemic sclerosis, SSc) is a fibrotic disease characterized by altered connective tissue and adhesion molecule expression.
  • Ectopeptidases play a role in regulating adhesion molecules and connective tissue turnover.

Purpose of the Study:

  • To investigate the expression and concentrations of three ectopeptidases in scleroderma patients compared to normal individuals.
  • To determine the role of these enzymes in the molecular pathology of SSc.

Main Methods:

  • Immunoperoxidase staining of skin sections.
  • Flow cytometry analysis of cultured dermal fibroblasts from SSc patients and controls.
  • Monoclonal antibodies were used to detect neutral endopeptidase-24.11 (NEP) (CD10), aminopeptidase N (APN) (CD13), and dipeptidyl peptidase IV (DPPIV) (CD26).

Related Experiment Videos

Main Results:

  • NEP (CD10) was not detected in skin tissue but was found in cultured fibroblasts.
  • APN (CD13) was detected in both SSc and normal skin and fibroblasts.
  • DPPIV (CD26) was present in lower concentrations in SSc skin and significantly downregulated in cultured SSc fibroblasts compared to controls.

Conclusions:

  • Dermal fibroblasts express cell surface proteases in vivo and in vitro.
  • The downregulation of DPPIV in SSc fibroblasts may contribute to increased adhesion molecules and matrix proteins.
  • DPPIV downregulation is implicated in the molecular pathology of scleroderma.