Related Experiment Videos
Molecular cloning of a novel human cDNA on chromosome 1q21 and its mouse homolog encoding a nuclear protein with
I Horikawa1, H Tanaka, Y Yuasa
1Department of Molecular and Cell Genetics, School of Life Sciences, Faculty of Medicine, Tottori University, Yonago, Japan.
Abstract:
We previously reported that human chromosome 1q21 or 1q23-q24 carries a transformation suppressor gene(s) for Kirsten sarcoma virus-transformed NIH3T3 cells. In this study, we have isolated a novel human cDNA on 1q21 (designated as YL-1) as a candidate, then cloned its mouse homolog. Human and mouse YL-1 cDNA shared 96% in amino acid homology and presumably encoded a transcription factor-like polypeptide. An analysis using a specific antibody revealed the nuclear localization of YL-1 protein. The bacterially expressed YL-1 could bind to DNA. Thus, YL-1 protein possibly functions as a transcriptional regulator.
Insights
Researchers identified a novel gene, YL-1, on human chromosome 1q21. This gene, found in both humans and mice, encodes a protein that regulates gene transcription by binding to DNA.
Area of Science:
- Genetics
- Molecular Biology
- Oncology
Background:
- Human chromosome 1q21 or 1q23-q24 was previously identified as carrying transformation suppressor genes.
- These genes are crucial for regulating the transformation of NIH3T3 cells by Kirsten sarcoma virus.
Purpose of the Study:
- To isolate and characterize a novel candidate transformation suppressor gene from human chromosome 1q21.
- To investigate the function and localization of the identified gene product.
Main Methods:
- Isolation of a novel human cDNA (YL-1) from chromosome 1q21.
- Cloning of the mouse homolog of YL-1.
- Analysis of amino acid homology between human and mouse YL-1.
- Western blot analysis using a specific antibody to determine protein localization.
- DNA-binding assays with bacterially expressed YL-1 protein.
Main Results:
- A novel human cDNA, YL-1, was isolated from chromosome 1q21.
- Human and mouse YL-1 cDNA exhibited 96% amino acid homology.
- YL-1 protein was found to be localized in the nucleus.
- Bacterially expressed YL-1 protein demonstrated DNA-binding capability.
Conclusions:
- YL-1 is a conserved gene in humans and mice, located on chromosome 1q21.
- The YL-1 protein functions as a transcriptional regulator, likely through its DNA-binding activity.
- YL-1 is a potential candidate for the transformation suppressor gene(s) on chromosome 1q21.