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Related Experiment Videos

Pathogenic potential of lactobacilli

D W Harty1, H J Oakey, M Patrikakis

  • 1Institute of Dental Research, Surry Hills, N.S.W., Australia.

International Journal of Food Microbiology
|December 1, 1994
PubMed
Summary
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Lactobacilli can cause diseases like infective endocarditis (IE) by aggregating platelets and binding to collagen. Understanding these pathogenic traits is crucial for safe probiotic use.

Area of Science:

  • Microbiology
  • Pathogenesis
  • Probiotics

Background:

  • Lactobacilli are generally beneficial but can cause diseases like infective endocarditis (IE).
  • Identifying pathogenic traits is key for safe probiotic applications.
  • Platelet aggregation by lactobacilli is a potential virulence factor in IE.

Purpose of the Study:

  • To investigate the pathogenic potential of Lactobacillus species.
  • To compare the ability of different Lactobacillus strains to aggregate human platelets.
  • To examine the binding of lactobacilli to host matrix proteins, including collagen.

Main Methods:

  • Assessed platelet aggregation by various Lactobacillus strains, including IE isolates.
  • Compared cell surface properties of Lactobacillus rhamnosus and Lactobacillus paracasei subsp. paracasei.

Related Experiment Videos

  • Investigated the effect of pH on fibronectin and fibrinogen binding.
  • Examined lactobacilli binding to different types of collagen.

    • Main Results:

    • Lactobacillus rhamnosus and other species demonstrated platelet aggregation, with IE strains showing higher aggregation.
    • Lactobacilli binding to fibronectin and fibrinogen increased significantly at lower pH.
    • Lactobacilli, especially IE-associated L. rhamnosus strains, showed enhanced binding to collagen types I and V.

    Conclusions:

    • Platelet aggregation and collagen binding are significant pathogenic traits in certain Lactobacillus species.
    • L. rhamnosus strains from IE cases exhibit increased binding to collagen types I and V, suggesting a role in heart valve colonization.
    • Findings aid in differentiating pathogenic from probiotic Lactobacillus strains.