Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Quantitative analysis of comparative genomic hybridization

S du Manoir1, E Schröck, M Bentz

  • 1Abteilung Organisation komplexer Genome, Deutsches Krebsforschungszentrum, Heidelberg, Germany.

Cytometry
|January 1, 1995
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

17.6% of patients in a German cohort with exocrine pancreatic cancer were diagnosed with a genetic tumor syndrome-a case for universal genetic testing?

ESMO gastrointestinal oncology·2026
Same author

Integrative multi-omics reveals a regulatory and exhausted T-cell landscape in CLL and identifies galectin-9 as an immunotherapy target.

Nature communications·2025
Same author

<i>Bordetella pertussis</i> exhibits genomic diversity within patients and laboratory culture.

mSystems·2025
Same author

Comprehensive genomic and transcriptomic analysis enables molecularly guided therapy options in peritoneal and pleural mesothelioma.

ESMO open·2025
Same author

Randomized phase-III study of low-dose cytarabine and etoposide + /- all-trans retinoic acid in older unfit patients with NPM1-mutated acute myeloid leukemia.

Scientific reports·2023
Same author

COGNITION: a prospective precision oncology trial for patients with early breast cancer at high risk following neoadjuvant chemotherapy.

ESMO open·2022

This study presents a new program for quantitative comparative genomic hybridization (CGH) analysis, improving the detection of chromosomal imbalances and defining rearrangement breakpoints for better genomic insights.

Area of Science:

  • Molecular Cytogenetics
  • Genomics

Background:

  • Comparative genomic hybridization (CGH) is a molecular cytogenetic technique used to detect chromosomal imbalances.
  • Accurate quantification of CGH data is essential for identifying subtle copy number variations and defining breakpoints in rearrangements.

Purpose of the Study:

  • To present a novel software program for the quantitative analysis of CGH preparations.
  • To establish objective parameters and criteria for evaluating CGH data quality.

Main Methods:

  • Developed a program to extract and average fluorescence ratio profiles along chromosomes from multiple metaphase spreads.
  • Assessed critical parameters like chromosome painting granularity and fluorescence intensity homogeneity.
  • Introduced the coefficient of variation of the ratio value for chromosomes in a balanced state (CVBS) as a quality metric.

Related Experiment Videos

Main Results:

  • The program enables quantitative evaluation of CGH experiments, crucial for detecting low copy changes (e.g., monosomy, trisomy).
  • Identified chromosome painting granularity and regional fluorescence inhomogeneity as key factors affecting data quality.
  • Demonstrated the utility of CVBS as a general quality criterion for CGH experiments.

Conclusions:

  • The developed program enhances the quantitative analysis of CGH, leading to more precise detection of chromosomal abnormalities.
  • Objective quality assessment criteria, including CVBS, improve the reliability and reproducibility of CGH studies.
  • This quantitative approach facilitates accurate identification of genomic imbalances and rearrangement breakpoints.