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cDNA cloning of a NGFI-B/nur77-related transcription factor from an apoptotic human T cell line
T Okabe1, R Takayanagi, K Imasaki
1Third Department of Internal Medicine, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Abstract:
A human T lymphoid cell line, PEER, dies by apoptosis in the presence of PMA and calcium ionophore. A new gene, TINUR, was cloned from apoptotic PEER cells. The expression of the TINUR gene is induced within 1 h after the cross-linking of the T cell Ag receptor complex. TINUR belongs to the NGFI-B/nur77 family of the steroid receptor superfamily and is an orphan receptor. TINUR binds to the same DNA sequence as NGFI-B/nur77. We also propose that the NGFI-B/nur77 family can be classified into two subtypes.
Insights
A new gene, TINUR, was identified in apoptotic T cells. TINUR gene expression is rapidly induced by T cell receptor activation, suggesting its role in T cell apoptosis.
Area of Science:
- Immunology
- Molecular Biology
- Genetics
Background:
- Human T lymphoid cell lines undergo apoptosis when stimulated with PMA and calcium ionophore.
- Apoptosis, or programmed cell death, is a critical process in immune system regulation.
Purpose of the Study:
- To identify novel genes involved in T cell apoptosis.
- To characterize the function and classification of the newly discovered TINUR gene.
Main Methods:
- Cloning of the TINUR gene from apoptotic PEER cells.
- Analysis of TINUR gene expression following T cell receptor complex cross-linking.
- Comparison of TINUR's DNA binding properties with known members of the NGFI-B/nur77 family.
Main Results:
- A novel gene, TINUR, was successfully cloned from apoptotic T lymphoid cells.
- TINUR gene expression is rapidly induced (within 1 hour) upon T cell receptor activation.
- TINUR, an orphan receptor belonging to the NGFI-B/nur77 family, shares DNA binding specificity with NGFI-B/nur77.
Conclusions:
- The TINUR gene is a novel, rapidly induced gene involved in T cell apoptosis.
- The findings support the classification of the NGFI-B/nur77 family into two distinct subtypes based on their characteristics.