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Cloning, sequence analysis and expression of two forms of mRNA coding for the human beta 2 subunit of the GABAA
D D McKinley1, D J Lennon, D B Carter
1Upjohn Company, Kalamazoo, MI 49001.
Brain Research. Molecular Brain Research
|January 1, 1995
Abstract:
Two forms of cDNA coding for the human GABAA beta 2 subunit have been cloned and sequenced. The two sequences differ by a 114 base pair insertion. The insert contains a phosphorylation consensus sequence for calmodulin-dependent protein kinase II. Quantitative PCR studies show that h beta 2L cDNA represents 10-15% of total h beta 2 cDNA in the 10 brain substructures tested. Analysis of human genomic southern blots suggests that the two forms might arise by differential splicing.