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Detection of Bacteroides fragilis endotoxin in amniotic fluid by counterimmunoelectrophoresis
I Beckmann1, K de Graaff, F Meisel-Mikołajczyk
1Department of Obstetrics and Gynecology, Erasmus University Medical School, Rotterdam, The Netherlands.
Antonie Van Leeuwenhoek
|January 1, 1994
Abstract:
The ability of counter immunoelectrophoresis (CIE) to detect Bacteroides fragilis endotoxin in amniotic fluid in small concentrations was evaluated. A method was developed which, in combination with ultrafiltration, permits detection of B. fragilis endotoxin in amniotic fluid in a concentration of 40 ng/ml or more. The sensitivity threshold was reduced to 2 ng/ml by using a highly reactive IgG-fraction isolated from rabbit anti-B. fragilis IPL E 323 antiserum.