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Updated: Aug 9, 2026

Simple and Robust in vivo and in vitro Approach for Studying Virus Assembly
Published on: March 1, 2012
Primary structure and sequence analysis of RNA2 of a mechanically transmitted barley mild mosaic virus isolate: an
J T Dessens1, M Nguyen, M Meyer
1Station de Pathologie Végétale, INRA Versailles, France.
Abstract:
The RNA2 nucleotide sequence of a mechanically transmitted isolate of barley mild mosaic virus (BaMMV) has been determined. A combination of Northern blot and sequence analysis indicates that this RNA2 lacks approximately 1000 nucleotides of its C-terminal protein (P2) gene, with respect to Polymyxa graminis transmitted BaMMV. This is confirmed by sequence comparison with RNA2 of a fungus transmitted BaMMV isolate, which reveals the presence of a single deletion located in the 3'-terminal part of the P2 gene. As a consequence, this RNA2 codes for a P2 protein of only 34 K. Sequence homology between the bymovirus P2 protein and the capsid readthrough protein of beet necrotic yellow vein virus and soil-borne wheat mosaic virus suggests an evolutionary relationship between bymo- and furoviruses.
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