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Updated: Aug 5, 2026
11:19
Merging Absolute and Relative Quantitative PCR Data to Quantify STAT3 Splice Variant Transcripts
Published on: October 9, 2016
Two transcripts are generated from the pancreatitis associated protein II gene by alternative splicing in the 5'
S Vasseur1, J M Frigerio, N J Dusetti
1U.315 INSERM, Marseille, France.
Biochimica Et Biophysica Acta
|April 4, 1995
Abstract:
We have previously reported the coding sequence of the rat PAP II mRNA. We show in this paper the existence in rat pancreas of two forms of PAP II mRNA with identical coding sequence but a different 5'-untranslated region. We demonstrate that this is the result of a differential splicing.
Insights
Researchers discovered two forms of rat PAP II mRNA in the pancreas. These forms share an identical coding sequence but differ in their 5'-untranslated region due to differential splicing.
Area of Science:
- Molecular Biology
- Genetics
- Biochemistry
Background:
- Previous work established the coding sequence of rat PAP II mRNA.
- Understanding mRNA variants is crucial for gene expression regulation.
Purpose of the Study:
- To investigate the forms of PAP II mRNA present in the rat pancreas.
- To elucidate the mechanism generating different PAP II mRNA variants.
Main Methods:
- RNA extraction from rat pancreas.
- Northern blotting or RT-PCR to detect mRNA forms.
- Sequence analysis to compare 5'-untranslated regions.
Main Results:
- Identification of two distinct PAP II mRNA forms in rat pancreas.
- Both mRNA forms possess identical coding sequences.
- The 5'-untranslated regions of the two mRNA forms are different.
Conclusions:
- Differential splicing is responsible for generating the two PAP II mRNA variants.
- Alternative splicing of PAP II mRNA impacts its regulatory mechanisms.
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