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Related Experiment Videos

Implementing transgenic and embryonic stem cell technology to study gene expression, cell-cell interactions and gene

S A Camper1, T L Saunders, S K Kendall

  • 1Department of Human Genetics, University of Michigan Medical School, Ann Arbor 48109-0618, USA.

Biology of Reproduction
|February 1, 1995
PubMed
Summary

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Transgenic mice and gene targeting revolutionized reproductive biology research. These technologies enable detailed studies of gene function, disease modeling, and cell lineage, advancing our understanding of physiology and disease.

Area of Science:

  • Reproductive Biology
  • Genetics
  • Animal Models

Background:

  • Transgenic animal technology, since 1980, has significantly advanced physiological studies.
  • Gene expression in whole animals requires complex DNA sequences, often necessitating transgenic models.
  • Embryonic stem (ES) cell technology, since 1987, enables targeted gene alterations in mice.

Purpose of the Study:

  • To review the applications of transgenic mice and gene targeting in reproductive biology.
  • To highlight their role in studying pituitary gene expression and cell lineage.
  • To demonstrate their utility in disease modeling and gene therapy testing.

Main Methods:

  • Utilizing transgenic mice to study gene expression and physiological effects.
  • Employing gene targeting with embryonic stem (ES) cells to create specific gene alterations.

Related Experiment Videos

  • Investigating cell-specific toxin gene expression for cell lineage studies.

    • Main Results:

    • Transgene expression profoundly impacts animal physiology and gene regulation.
    • Transgenic mice serve as valuable models for cancer, disease, and gene therapy.
    • Targeted gene disruption, like in the inhibin gene, revealed unexpected roles, such as tumor suppression.

    Conclusions:

    • Transgenic and ES cell technologies are crucial for understanding gene function in reproduction.
    • These technologies continue to expand knowledge and challenge existing paradigms in reproductive biology.
    • Future applications include transgene ablation experiments using inducible toxins.