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Related Experiment Videos

Xylene substitutes in frozen sections

J D Whalen1, R G Dufresne, C S Wilkel

  • 1Brown University School of Medicine, Department of Dermatology, Roger Williams Medical Center, Providence, Rhode Island, USA.

Dermatologic Surgery : Official Publication for American Society for Dermatologic Surgery [Et Al.]
|March 1, 1995
PubMed
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Safer xylene substitutes are as effective as xylene in Mohs laboratory histology. This study found no difference in staining quality, clarity, or tissue detail, supporting their adoption for improved safety.

Area of Science:

  • Histopathology
  • Dermatology
  • Laboratory Safety

Background:

  • Xylene exposure poses risks during histopathologic staining.
  • Safer xylene substitutes have been available for over 10 years.
  • A 1991 survey indicated limited adoption of xylene substitutes in Mohs training programs.

Purpose of the Study:

  • To compare the efficacy of xylene versus xylene substitutes.
  • To evaluate staining quality, clarity, cellular detail, and tissue distortion.
  • To assess safety and efficacy in the histologic preparation of frozen sections.

Main Methods:

  • Xylene and substitutes were used as clearing agents for fresh frozen horizontal sections.
  • Slides were prepared using routine histologic techniques.

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  • A blinded evaluation was conducted by a dermatopathologist and a Mohs surgeon.

    • Main Results:

    • No significant differences were observed between xylene and xylene substitutes.
    • Staining quality, clarity, and cellular detail remained consistent.
    • Tissue distortion was comparable across all tested agents.

    Conclusions:

    • Xylene substitutes offer an improved safety profile without compromising efficacy.
    • The study recommends replacing xylene with substitutes in Mohs laboratories.
    • Adoption of xylene substitutes can enhance laboratory safety for histopathologic procedures.