Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

What is important on the red blood cell surface

P Tippett1

  • 1Medical Research Council Blood Group Unit, University College London, UK.

Immunological Investigations
|January 1, 1995
PubMed
Summary

This study details a new technique, MAIEA (monoclonal antibody-specific immobilisation of erythrocyte antigen), for identifying blood group antigens and their locations on red blood cells. It confirms antigen locations and discusses variations in antigen expression.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Ruth Sanger.

Vox sanguinis·2002
Same author

The Xg blood group system: a review.

Transfusion medicine reviews·1998
Same author

The Rh antigen D: partial D antigens and associated low incidence antigens.

Vox sanguinis·1996
Same author

The D antigen characteristic of RoHar is a partial D antigen.

Vox sanguinis·1996
Same author

Rhmod phenotype: a parentage problem solved by denaturing gradient gel electorphoresis of genomic DNA.

Immunohematology·1996
Same author

Glycophorin A-deficient red cells may have a weak expression of C4-bound Ch and Rg antigens.

Immunohematology·1996

Area of Science:

  • Hematology
  • Immunogenetics
  • Molecular Biology

Background:

  • Blood group antigens are crucial for understanding red cell surface, genetics, and forensic studies.
  • Precise definition of red blood cell phenotypes is essential for accurate research and clinical applications.
  • Existing methods for antigen identification and localization require refinement for complex genetic systems.

Purpose of the Study:

  • To describe the application of a novel technique, MAIEA, for identifying red cell antigens.
  • To determine the precise membrane component localization of specific blood group antigen systems.
  • To investigate variability in red cell antigen expression and explore potential causes for Rh variant phenotypes.

Main Methods:

  • Utilized MAIEA (monoclonal antibody-specific immobilisation of erythrocyte antigen) for antigen identification.
  • Applied the technique to confirm the location of Knops system antigens on CR1.
  • Provisionally assigned Cromer system antigens to specific regions of decay accelerating factor (DAF).

Main Results:

  • Confirmed the location of Knops system antigens on the CR1 protein.
  • Provisionally assigned Cromer system antigens to short consensus regions of decay accelerating factor (DAF).
  • Described C variants, including two associated with low-incidence antigens, and reconsidered the Xga relationship with 12E7 antigen polymorphism.

Conclusions:

  • MAIEA is an effective technique for identifying red cell antigens and their membrane localization.
  • The study provides new insights into the genetic basis and expression of blood group antigen systems.
  • Further research into Rh variant phenotypes and antigen quantitative polymorphism is warranted.

Related Experiment Videos