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Quantification of adenovirus particles

H Liebermann¹, R Mentel

¹Institut für Medizinische Mikrobiologie, E.-M.-Arndt-Universität, Greifswald, Germany.

Journal of Virological Methods

|December 1, 1994

Summary

This study presents a rapid physical method for quantifying adenovirus using sucrose gradient centrifugation and UV photometry, yielding results in about an hour. The method accurately measures adenovirus yield and characteristics from cell cultures.

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Area of Science:

Virology
Biophysical Chemistry
Analytical Chemistry

Background:

Accurate quantification of adenovirus is crucial for research and diagnostics.
Existing methods may be time-consuming or lack precision.
A rapid and sensitive physical assay for adenovirus is needed.

Purpose of the Study:

To develop and validate a physical method for adenovirus quantification.
To compare the sensitivity of UV detection at different wavelengths.
To assess the method's utility in determining virus yield and characteristics.

Main Methods:

Centrifugation of adenovirus in a gentle sucrose gradient.
Analysis using a sensitive UV flow photometer.
Determination of virus mass (micrograms/ml) within approximately 1 hour.

Main Results:

The method successfully quantified adenovirus yield from FL-cell cultures (< 0.1 to 7 micrograms/ml).
Sensitivity was compared at UV wavelengths 254 nm, 278 nm, and 226 nm.
No significant difference was observed compared to CsCl density equilibrium ultracentrifugation.

Conclusions:

The developed physical method offers a rapid and reliable approach for adenovirus quantification.
The technique allows for the determination of virus yield and other physical properties.
This method provides an efficient alternative to traditional ultracentrifugation techniques.

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