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[Use of collase for culturing cells]
Voprosy Virusologii
|November 1, 1994
Summary
Collase, an enzyme from crab hepatopancreas, enhances cell culture by improving cell harvest and preserving cell health better than trypsin. This enzyme is ideal for primary cell cultures and cell detachment during reinoculation.
Area of Science:
- Biochemistry
- Cell Biology
- Enzymology
Context:
- Cell culture techniques are crucial for biological research and drug development.
- Enzymatic reagents are commonly used for cell detachment and dissociation.
- Trypsin is a widely used but often harsh enzymatic reagent.
Purpose:
- To evaluate Collase, a crab hepatopancreas enzymatic preparation, as a dissociative reagent.
- To compare the efficacy and cellular impact of Collase with trypsin.
- To assess Collase's suitability for primary cell culture and continuous cell line maintenance.
Summary:
- Collase, derived from crab hepatopancreas, effectively dissociates cells for culture preparation.
- It increased viable cell harvest by 2 to 2.5 times compared to trypsin.
- Collase demonstrated a less detrimental effect on cell proliferative activity, morphology, productivity, and viral sensitivity.
Impact:
- Collase offers a gentler and more effective alternative to trypsin for cell dissociation.
- Improved cell viability and preserved cellular functions can enhance research outcomes.
- This enzymatic preparation has potential applications in various cell-based assays and bioproduction.