Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Fast-scan cyclic voltammetry of 5-hydroxytryptamine

B P Jackson1, S M Dietz, R M Wightman

  • 1Department of Chemistry, University of North Carolina at Chapel Hill 27599-3290.

Analytical Chemistry
|March 15, 1995
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Association between Mediterranean diet and metal mixtures concentrations in pregnant people from the New Hampshire Birth Cohort Study.

The Science of the total environment·2023
Same author

Supplementation of Ascophyllum nodosum meal and monensin: Effects on diversity and relative abundance of ruminal bacterial taxa and the metabolism of iodine and arsenic in lactating dairy cows.

Journal of dairy science·2022
Same author

Arsenic exposure in relation to apple consumption among infants in the New Hampshire Birth Cohort Study.

Exposure and health·2020
Same author

Contrasting arsenic cycling in strongly and weakly stratified contaminated lakes: Evidence for temperature control on sediment-water arsenic fluxes.

Limnology and oceanography·2019
Same author

Dissecting Kawasaki disease: a state-of-the-art review.

European journal of pediatrics·2017
Same author

Regression and Complications of z-score-Based Giant Aneurysms in a Dutch Cohort of Kawasaki Disease Patients.

Pediatric cardiology·2017
Same journal

Structural Hairpin Anchoring-Mediated TtAgo Activity Regulation for Programmable Biosensing.

Analytical chemistry·2026
Same journal

Digital Revitalization of a Legacy Linear Ion Trap System.

Analytical chemistry·2026
Same journal

An Interface-Regulated Electrochemical Biosensing Platform Based on the Cascade Amplification of Primer Exchange Reaction and CRISPR/Cas12a for Noninvasive Bladder Cancer Diagnosis.

Analytical chemistry·2026
Same journal

Spatially Resolved Diffusion NMR for Structurally Heterogeneous Materials.

Analytical chemistry·2026
Same journal

Direct Whole-Blood Multiplexing of Small Molecules via a Micelle-Enhanced Chemiluminescent Paper Sensor with Mesoporous Silica Membrane.

Analytical chemistry·2026
Same journal

Modeling the Effects of Short-Range Randomness in Packed Sphere Beds.

Analytical chemistry·2026
See all related articles

This study introduces a new fast-scan cyclic voltammetry method for detecting 5-hydroxytryptamine (5-HT) in vivo. The technique successfully differentiates 5-HT from other compounds and measures its uptake rate in rat brain tissue.

Area of Science:

  • Neuroscience
  • Analytical Chemistry
  • Electrochemistry

Background:

  • Fast-scan cyclic voltammetry (FSCV) is a validated method for in vivo dopamine detection.
  • 5-hydroxytryptamine (5-HT) detection in vivo is challenging due to slow electrode response times caused by 5-HT adsorption.

Purpose of the Study:

  • To extend FSCV for in vitro and in vivo detection of 5-HT.
  • To develop a waveform that overcomes 5-HT adsorption issues and enables rapid detection.

Main Methods:

  • A specific waveform (0.2 to 1.0 to -0.1 to 0.2 V) applied at 1000 V/s was used to accelerate electrode response.
  • Carbon fiber disk microelectrodes and Nafion-coated electrodes were employed.
  • In vivo measurements were conducted in the caudate of anesthetized rats.

Related Experiment Videos

Main Results:

  • The new waveform successfully outruns adsorption-related reactions, enabling rapid 5-HT detection.
  • Peak currents of 1 nA (bare electrode) and 5 nA (Nafion-coated) were observed for 1 microM 5-HT.
  • 5-HT could be differentiated from other compounds by peak potentials.
  • Cellular uptake of 5-HT was found to be 3-fold slower than dopamine uptake.

Conclusions:

  • Fast-scan cyclic voltammetry with a specialized waveform is effective for in vivo 5-HT detection.
  • Nafion-coated electrodes enhance 5-HT detection sensitivity.
  • The method allows for monitoring of neurotransmitter dynamics and comparison of uptake rates.