Related Experiment Video
Updated: Sep 4, 2026

Improved UPLC-UV Method for the Quantification of Vitamin C in Lettuce Varieties (Lactuca sativa L.) and Crop Wild Relatives (Lactuca spp.)
Published on: June 30, 2020
[Effect of ripening and drying on pro-vitamin A carotenoid content in pepper (Capsicum annuum var. Anaheim)]
S Y Moya1, L A Mejía, E González de Mejía
1Centro de Investigación en Alimentación y Desarrollo A.C. (CIAD, A.C.), Hermosillo, Sonora, México.
Abstract:
The content of the principal carotenes with biological activity (alpha-, beta, gamma-carotene and beta-criptoxantine) in green pepper (Capsicum annuum var. Anaheim) was determined in order to investigate the vitamin A content during the traditional process of ripening and drying. The analytical technique used was high pressure liquid chromatography (HPLC) with a Novapack C18 column and acetonitrile/methanol/tetrahydrofuran (58V:35V:7V) as a mobile phase. The results showed differences in vitamin A activity of 7.2 +/- 0.8, 51.1 +/- 2.4 and 55.3 +/- 1.2 micrograms of RE/g dry matter for immature, mature and dry pepper fruit respectively (p < 0.05). Approximately 95% of total activity was due to beta-carotene being synthesized faster than the other carotenes under study during the ripening process. Total vitamin A activity reached its highest value at 14 days after harvest (2738.8 +/- 128.6 micrograms RE/100 g of edible portion), with a decrease in activity with time.
More Related Videos
08:27Analysis of Effect of Compound Salt Stress on Seed Germination and Salt Tolerance Analysis of Pepper (Capsicum annuum L.)
Published on: November 30, 2022
08:45Formulation of Vitamin A Tracer Doses for Administration to Human Subjects using Retinol Isotope Dilution Technique
Published on: March 31, 2026
Related Concept Videos
Fruit Development, Structure, and Function
The Calvin Benson Cycle
Loss of Carboxy Group as CO2: Decarboxylation of Malonic Acid Derivatives