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Updated: Aug 14, 2026

Induction of Alloantigen-specific Anergy in Human Peripheral Blood Mononuclear Cells by Alloantigen Stimulation with Co-stimulatory Signal Blockade
Published on: March 14, 2011
Immunoaffinity measurement of recombinant granulocyte colony stimulating factor in patients with chemotherapy-induced
1Immunochemistry Laboratory, George Washington University Medical Center, Washington, D.C. 20037.
Abstract:
A high-performance immunoaffinity chromatographic technique has been developed for the measurement of recombinant human granulocyte colony stimulating factor in human patients receiving this agent, following neutropenia, arising from cancer chemotherapy. The technique employs a short, biocompatible polymer column packed with minute, antibody-coated glass beads. This system was applied to the analysis of recombinant human granulocyte colony stimulating factor in three different human body fluids. A reasonable degree of correlation was achieved when comparing the immunoaffinity technique to a conventional immunoassay, although the immunoaffinity technique displayed greater specificity.
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