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Clarification of PC12 cell growth inhibition by a sialoglycopeptide from bovine brain under low serum condition
1Department of Biological and Chemical Engineering, Faculty of Engineering, Gunma University, Japan.
Cell Structure and Function
|December 1, 1994
Abstract:
To study the effect of a bovine brain sialoglycopeptide (SGP) on the nerve cell growth, PC12 cells were used as a model system. Though the inhibition of cell growth, measured by the 3-(4,5-dimethylthiazol-2-yl) 2,5-diphenyltetrazolium bromide (MTT) assay, by SGP was slight under the serum concentration of 10%, it was clearly detected under that of 1 to 2%. For the preparation of SGP, filtration through a nitrocellulose membrane was useful.