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Updated: Aug 8, 2026
10:52
Radiolabeling and Quantification of Cellular Levels of Phosphoinositides by High Performance Liquid Chromatography-coupled Flow Scintillation
Published on: January 6, 2016
Nuclear phosphoinositide signalling enzyme in human B lymphoid cells
A Cataldi1, R Di Pietro, I Robuffo
1Istituto di Morfologia Umana Normale, Università G. D'Annunzio, Chieti, Italy.
Cell Structure and Function
|December 1, 1994
Summary
Interferon treatment increases phosphoinositidase C (PIC) beta activity in Burkitt lymphoma cells, suggesting nuclear PIC beta 1 recruitment mediates interferon's antiproliferative effects.
Area of Science:
- Cell Biology
- Molecular Biology
- Immunology
Background:
- Interferon (IFN) is crucial for antiviral and antitumor responses.
- Phosphoinositidase C (PIC) beta enzymes play roles in cellular signaling pathways.
- Understanding IFN's mechanism in Burkitt lymphoma is vital for therapeutic development.
Purpose of the Study:
- To investigate the effect of interferon on phosphoinositidase C (PIC) beta activity, expression, and localization in Burkitt lymphoma cells.
- To elucidate the role of nuclear PIC beta 1 in interferon's antiproliferative action.
Main Methods:
- Western blotting to analyze protein expression.
- Immunocytochemistry and immunoelectronmicroscopy for cellular localization.
- Analysis of phosphatidyl-inositol-bisphosphate (PIP2) hydrolysis.
Main Results:
- Interferon treatment led to increased PIP2 hydrolysis at the nuclear level in Daudi cells.
- PIC beta 1 expression increased and was detected in the nuclear compartment upon IFN treatment.
- A resistant cell line showed no modulation of PIC beta 1 activity or PIP2 hydrolysis upon IFN treatment.
Conclusions:
- Interferon's antiproliferative effect on Burkitt lymphoma cells may involve the recruitment of nuclear PIC beta 1.
- Nuclear PIC beta 1 activity is a potential target for enhancing interferon therapy in lymphoma.
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