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Apoptosis by demecolcine in V79 cells
K Fujikawa-Yamamoto1, K Teraoka, Z P Zong
1Division of Basic Science, Kanazawa Medical University, Ishikawa, Japan.
Cell Structure and Function
|December 1, 1994
Summary
Demecolcine (Colcemid) induces apoptosis in V79 cells by inhibiting spindle fiber formation. Higher concentrations lead to hyperploidy, while lower doses show temporal M phase accumulation before cell death.
Area of Science:
- Cell Biology
- Molecular Biology
- Toxicology
Background:
- Demecolcine (Colcemid) is a known inhibitor of spindle fiber formation.
- Understanding the cellular response to spindle disruptors is crucial in cell cycle research.
Purpose of the Study:
- To investigate the effects of Demecolcine on V79 cell proliferation and cell death.
- To determine the concentration-dependent cytotoxic effects of Demecolcine.
Main Methods:
- V79 cells were treated with varying concentrations of Demecolcine.
- Cell proliferation was assessed.
- Cell cycle progression was monitored via M phase accumulation.
- Apoptosis was quantified using DNA histograms (sub-G1 peak) and gel electrophoresis (DNA fragmentation).
Main Results:
- At 0.01 µg/ml, Demecolcine allowed exponential cell proliferation with transient M phase arrest.
- Concentrations of 0.1 µg/ml resulted in hyperploid V79 cells.
- Apoptosis was induced at 0.03 µg/ml Demecolcine, evidenced by DNA fragmentation and a sub-G1 peak.
Conclusions:
- Demecolcine induces apoptosis in V79 cells at specific concentrations.
- The drug exhibits dose-dependent effects on cell cycle progression, including hyperploidy and cell death.