[An approach for PCR amplification of long DNA fragments]

The polymerase chain reaction (PCR) technique, evolved as a standard laboratory technique, has a major limitation: its inability to efficiently amplify fragments higher than 1.5 kb. In this paper, PCR conditions allowing the efficient amplification of long DNA fragments, especially a new proper buffer system, have been developed. The difference between the new PCR reaction buffer system and standard reaction system is that no KCl is included in the former. The results indicated that specificity and reproducibility for amplifying long DNA fragments by using new buffer system are satisfactory.