[Isolation and purification of glyceraldehyde-3-phosphate dehydrogenase from Schistosoma japonicum]

This paper recommends a simple and easy method to isolate and purify glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from Schistosoma japonicum with electrophoretic purity. The recovery rate of 56.1% of glyceraldehyde-3-phosphate dehydrogenase from S. japonicum extract was achieved by ammonium sulfate precipitation, DEAE-cellulose column chromatography and SDS-PAGE. Using SDS-PAGE, the presence of one major band in the final purified GAPDH preparation was demonstrated. This protein had a MW of 37 kDa. The authors discuss the important functions of GAPDH and suggest that this method may be useful in the research of the protective immunity of S. japonicum GAPDH in the development of schistosome vaccine.