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Urinary 6 beta-hydroxycortisol in humans: analysis, biological variations, and reference ranges

C Lee1

  • 1Department of Medical Technology, School of Allied Health Sciences, University of Texas Medical Branch, Galveston 77555-1028, USA.

Clinical Biochemistry
|February 1, 1995
PubMed
Summary

This study validates a method for measuring 6 beta-hydroxycortisol (6 beta-OHF) in urine, establishing reference ranges and demonstrating its stability. An early morning urine sample is sufficient for assessing the 6 beta-OHF to urinary free cortisol ratio.

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Area of Science:

  • Clinical Chemistry
  • Endocrinology
  • Analytical Chemistry

Background:

  • 6 beta-hydroxycortisol (6 beta-OHF) is a metabolite of cortisol.
  • Accurate measurement of 6 beta-OHF is important for assessing adrenal function.
  • Establishing reliable reference ranges and analytical methods is crucial for clinical application.

Purpose of the Study:

  • To evaluate analytical methods for urinary 6 beta-hydroxycortisol.
  • To determine biological variation and reference ranges for urinary 6 beta-hydroxycortisol.
  • To assess the utility of early morning urine samples for 6 beta-OHF measurements.

Main Methods:

  • Urinary 6 beta-hydroxycortisol (6 beta-OHF) was measured using reversed-phase high-performance liquid chromatography.
  • Assay accuracy and precision were assessed through recovery rates and coefficients of variation.

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  • Intra-individual biological variation and diurnal rhythms were analyzed over a 5-day period.

    • Main Results:

    • The HPLC method demonstrated high accuracy (92-95% recovery) and precision (<5.5% within-run, <6.6% between-run CV).
    • Reference ranges for urinary 6 beta-OHF were established: 187-1245 nmol/day for adults and 103-915 nmol/day for the elderly.
    • Urinary 6 beta-OHF and free cortisol showed parallel diurnal rhythms, maintaining a constant ratio throughout the day.

    Conclusions:

    • The validated HPLC method provides accurate and precise measurements of urinary 6 beta-hydroxycortisol.
    • Established reference ranges aid in the interpretation of 6 beta-OHF levels in different age groups.
    • An early morning urine specimen is adequate for determining the urinary 6 beta-OHF to free cortisol ratio, simplifying sample collection.