Related Experiment Videos
Observation of the pre-steady state process in thermolysin catalysis with a fluorescent displacement probe at low pH
S Kunugi1, M Yokoyama, A Sakamoto
1Department of Polymer Science and Engineering, Kyoto Institute of Technology, Japan.
FEBS Letters
|April 3, 1995
Abstract:
The pre-steady state process in the thermolysin-catalyzed hydrolysis of Cbz-Gly-Phe-Ala was observed at pH 4.5 by fluorescence stopped-flow method using Dns-Phe as a displacement probe. After the confirmation of the pre-equilibrium hypothesis for the binary interaction, the nonlinear substrate concentration dependence of the apparent kinetic constant for the pre-steady state process was analyzed and an existence of multi-intermediates was proposed.