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Construction of a human DNA library in a circular centromere-based yeast plasmid
T McGonigal1, P Bodelle, C Schopp
1Pharmaceutical Products Division, Abbott Laboratories, Abbott Park, IL 60064, USA.
Gene
|April 3, 1995
Abstract:
The construction of a human DNA library in a centromere-based circular yeast plasmid is described. The vector contains the yeast CEN3 sequence, the URA3 gene for propagation in yeast and a hygromycin-resistance gene (HyR) for selection in mammalian cells. The library consists of 64,000 members with an average insert size of 150 kb, with some members containing inserts of > 1 Mb. We calculate that the library contains three human genome equivalents of DNA. Clones can be identified by a PCR-based screening of DNA pools from individual colonies that have been stored in microtiter wells.