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A stationary-phase-dependent viability block governed by two different polypeptides from the RhsA genetic element of
1Department of Biochemistry and Molecular Biology, Milton S. Hershey Medical Center, Pennsylvania State University, Hershey 17033, USA.
Journal of Bacteriology
|April 1, 1995
Abstract:
Multicopy plasmids bearing a small internal portion of the RhsA genetic element of Escherichia coli K-12 imparted a viability block on cultures grown to stationary phase in broth. Inclusion of the last 25 codons of the RhsA core open reading frame (called core-ORF) in the plasmid insert was crucial for eliciting this toxic effect. The toxic effect could be suppressed by including the adjacent Rhs component, dsORF-a1, on the multicopy plasmid. The toxic effect was enhanced in RpoS- strains.