Related Experiment Videos
[Fundamental and practical study for DNA analysis using tooth as a source of DNA]
1Department of Forensic Odontology, Tokyo Dental College, Chiba, Japan.
Nihon Hoigaku Zasshi = the Japanese Journal of Legal Medicine
|February 1, 1995
Summary
High molecular weight DNA can be extracted from dental pulp even after 336 days of room temperature storage. This DNA is suitable for forensic analysis, including DNA fingerprinting and PCR.
Area of Science:
- Forensic Science
- Molecular Biology
- Genetics
Context:
- Dental tissues are a valuable source of DNA for forensic identification.
- Investigating DNA extraction methods and degradation is crucial for reliable forensic analysis.
- Tooth samples are often stored under various conditions before analysis.
Purpose:
- To evaluate DNA yield and degradation from dental pulp stored at room temperature.
- To optimize DNA extraction methods from tooth hard tissues.
- To assess the suitability of extracted DNA for forensic applications like DNA fingerprinting and PCR analysis.
Summary:
- DNA extraction from dental pulp yielded 3-40 micrograms of high molecular weight DNA, even after 336 days at room temperature.
- Efficient DNA extraction from tooth hard tissues was achieved using EDTA decalcification.
- DNA from dental pulp and hard tissues allowed for identical DNA fingerprinting and D4S43 typing, confirming its forensic utility.
Impact:
- Establishes optimal methods for DNA extraction from dental tissues for forensic use.
- Demonstrates the long-term stability of DNA in stored tooth samples.
- Provides a reliable DNA source for forensic identification, even from degraded samples.