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Microwave applications on small insect brain tissue irradiated in a water-perfused cooling-jacket for temperature
1Department of Entomology, Wageningen Agricultural University, The Netherlands.
Journal of Neuroscience Methods
|December 1, 1994
Abstract:
This paper describes a technique for microwave-accelerated fixation, washing and pre-embedding immunohistochemistry of insect brains. The temperature of the specimens is controlled with a water-cooling device. This device also serves as a permanent water load for microwave energy absorption and as a stirrer to avoid uneven energy distribution. The set-up described here allows continuous irradiation of specimens at full power, for unlimited time, without overheating. A considerable reduction in processing time could thus be achieved, along with improved immunolabelling.