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Related Experiment Videos

Extraction of lipids from yeast

M T Sobus, C E Homlund

    Lipids
    |April 1, 1976
    PubMed
    Summary

    Researchers compared lipid extraction methods for yeast cells. Methanol and benzene extraction efficiently recovered polar and nonpolar lipids, offering a superior alternative for lipid analysis.

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    Effects of the hypocholesteremic agent trifluperidol on the sterol, steryl ester, and fatty acid metabolism of Saccharomyces cerevisiae.

    Journal of bacteriologyยท1977
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    Area of Science:

    • Biochemistry
    • Microbiology
    • Analytical Chemistry

    Background:

    • Lipid extraction from microbial cells is crucial for understanding cellular function and composition.
    • Optimizing extraction methods is essential for accurate lipidomic studies.
    • Yeast (Saccharomyces cerevisiae) is a model organism for studying eukaryotic cell biology and lipid metabolism.

    Purpose of the Study:

    • To compare the efficiency of different lipid extraction methods from intact yeast cells.
    • To identify the optimal solvent system for maximizing lipid recovery.
    • To evaluate a preparative method for enhanced steryl ester extraction.

    Main Methods:

    • Comparison of various solvent extraction techniques on intact yeast cells.
    • Solvent systems evaluated included methanol, methanol:benzene (1:1, v/v), and benzene.
    • A preparative method involving aqueous potassium hydroxide (KOH) preincubation was assessed.

    Main Results:

    • Extraction with methanol followed by methanol:benzene and benzene yielded equal or greater amounts of polar and nonpolar lipids compared to other methods.
    • The preparative method with KOH preincubation showed slightly higher recovery of steryl esters than extraction from broken cells.
    • This indicates that intact cell extraction can be highly effective.

    Conclusions:

    • Methanol and benzene-based solvent systems are highly effective for extracting lipids from intact yeast cells.
    • The described preparative method offers an advantage for isolating steryl esters.
    • These findings provide an optimized protocol for yeast lipid analysis.

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