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Updated: Aug 19, 2026

Dissection of Saccharomyces Cerevisiae Asci
Published on: May 19, 2009
The Saccharomyces cerevisiae processing alpha 1,2-mannosidase is an inverting glycosidase
F Lipari1, B J Gour-Salin, A Herscovics
1McGill Cancer Centre, McGill University, Montréal, Québec, Canada.
Abstract:
The alpha 1,2-mannosidase from Saccharomyces cerevisiae, which removes one specific alpha 1,2-linked mannose residue from Man9GlcNAc2, is a member of the Class 1 alpha 1,2-mannosidase family conserved from yeast to mammals. Although Class 1 alpha 1,2-mannosidases are essential for the maturation of N-linked oligosaccharides in mammalian cells, nothing is known about their mechanism of action. The availability of sufficient quantities of recombinant yeast alpha 1,2-mannosidase and its homology with the mammalian enzymes make it a good model to study the catalytic mechanism of this family of alpha 1,2-mannosidases. The stereochemical course of hydrolysis of Man9GlcNAc by the yeast enzyme was followed by proton nuclear magnetic resonance spectroscopy. It was observed that beta-D-mannose is related from the oligosaccharide substrate, thereby demonstrating that the enzyme is of the inverting type.
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