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Enzymatic amplification and molecular cloning of cDNA encoding the small and large subunits of bovine interleukin 12
D S Zarlenga1, A Canals, R A Aschenbrenner
1US Department of Agriculture, Agricultural Research Service, Parasite Immunobiology Lab, Beltsville, MD 20705, USA.
Biochimica Et Biophysica Acta
|April 24, 1995
Abstract:
cDNA generated from stimulated abomasal lymph node cells was used to amplify and clone the 35 kDa and 40 kDa subunits of bovine interleukin 12 (IL-12) using primers derived from semi-conserved regions between human and mouse IL-12 sequences. The deduced amino acid sequence of the 40 kDa subunit demonstrated 84.4% and 67.6% homology with human and mouse sequences, respectively. The deduced sequence of the 35 kDa subunit exhibited comparable similarities to the human 35 kDa subunit (82.2%) but differed significantly (58.6%) from mouse-derived sequences.