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Related Experiment Videos

Vitamin C and glycohemoglobin

C W Weykamp1, T J Penders, H Baadenhuijsen

  • 1Department of Clinical Chemistry, Queen Beatrix Hospital, Winterswijk, The Netherlands.

Clinical Chemistry
|May 1, 1995
PubMed
Summary

High-dose vitamin C supplementation in nondiabetics does not affect hemoglobin glycation (GHb) measurements. This study found no interference with common GHb testing methods, ensuring accurate results.

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Area of Science:

  • Biochemistry
  • Clinical Chemistry
  • Nutritional Science

Background:

  • Glycohemoglobin (GHb) is a key indicator of long-term glycemic control.
  • Vitamin C (ascorbic acid) is an antioxidant with potential effects on blood glucose metabolism.
  • Previous research has yielded conflicting results regarding vitamin C's impact on GHb levels.

Purpose of the Study:

  • To investigate the effect of daily vitamin C supplementation on GHb levels in nondiabetic individuals.
  • To determine if vitamin C interferes with common GHb measurement techniques.
  • To assess the impact of vitamin C on in vivo hemoglobin glycation.

Main Methods:

  • A randomized controlled trial involving 30 nondiabetic volunteers.
  • Supplementation with 0, 750, or 1500 mg of vitamin C daily for 12 weeks.

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  • Measurement of GHb using HPLC, electrophoresis, affinity chromatography, and immunoassay at multiple time points.

    • Main Results:

    • Plasma vitamin C levels increased significantly with supplementation.
    • No significant differences in GHb, glucose, or fructosamine concentrations were observed between groups.
    • Vitamin C supplementation did not interfere with any of the GHb assay methods used.

    Conclusions:

    • Daily supplementation with 750 or 1500 mg of vitamin C for 12 weeks does not affect GHb levels in nondiabetics.
    • Vitamin C does not interfere with the accuracy of GHb determination by common laboratory methods.
    • The study does not support a role for vitamin C in reducing in vivo Hb glycation.