Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A cellulose acetate immunofixation technique

J Kohn, P G Riches

    Journal of Immunological Methods
    |January 1, 1978
    PubMed
    Summary
    This summary is machine-generated.

    This study introduces a rapid, sensitive, and economical immunofixation electrophoresis method using cellulose acetate membrane (CAM) for protein analysis. The technique aids in identifying paraproteins and analyzing biological fluids like in phenotyping.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Intracorporeal or extracorporeal anastomosis after minimally invasive right colectomy: a systematic review and meta-analysis.

    Techniques in coloproctology·2023
    Same author

    Structure and thermal transitions in a biomedically relevant liquid crystalline poly(ester amide).

    Macromolecules·2019
    Same author

    Evaluation of early clinical failure criteria for gram-negative bloodstream infections.

    Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases·2019
    Same author

    Achieving molecular orientation in thermally extruded 3D printed objects.

    Biofabrication·2019
    Same author

    A Standardized Approach to Cesarean Surgical Technique and Its Effect on Operative Time and Surgical Morbidity.

    American journal of perinatology·2018
    Same author

    Cumulative Effect of an Antimicrobial Stewardship and Rapid Diagnostic Testing Bundle on Early Streamlining of Antimicrobial Therapy in Gram-Negative Bloodstream Infections.

    Antimicrobial agents and chemotherapy·2017
    Same journal

    Optimized intracellular flow cytometry panel enables CD4 and CD8 T cell cytokine profiling in Syrian hamsters.

    Journal of immunological methods·2026
    Same journal

    Isosulfan blue sentinel lymph node biopsy enables reliable lymph node harvest and multicolor flow cytometry in mice.

    Journal of immunological methods·2026
    Same journal

    Type-specific antibody detection of herpes simplex virus types 1&2 (HSV-1&2) in fingerstick blood at point-of-care sites by a rapid and sensitive lateral flow immunochromatographic assay.

    Journal of immunological methods·2026
    Same journal

    Development of chimeric DGP-IgG antibodies as quality control for celiac disease diagnosis.

    Journal of immunological methods·2026
    Same journal

    Stepwise single-cell-resolved deep immunophenotyping pipeline to characterise immune heterogeneity and functionality in health and disease.

    Journal of immunological methods·2026
    Same journal

    Performance evaluation of Meso Scale Discovery (MSD) quantitative serological assays for detection of binding (IgG, IgA, IgM) and ACE2 inhibitory antibody levels for SARS-CoV-2.

    Journal of immunological methods·2026
    See all related articles

    Area of Science:

    • Clinical Chemistry
    • Immunology
    • Protein Electrophoresis

    Background:

    • Immunofixation electrophoresis is crucial for identifying and characterizing proteins in biological samples.
    • Conventional methods can be time-consuming and require specialized equipment.

    Purpose of the Study:

    • To develop a simplified and efficient immunofixation method.
    • To enhance the detection and classification of paraproteins and analyze other biological fluid proteins.

    Main Methods:

    • Proteins are separated using conventional electrophoresis on cellulose acetate membrane (CAM).
    • Brief immersion in specific antiserum diluted with barbitone buffer and polyethylene glycol (PEG).
    • Washing and staining with Ponceau S or Nigrosin.

    Related Experiment Videos

    Main Results:

    • The method is fast, sensitive, and economical, requiring no expensive reagents or equipment.
    • Successfully identified minor paraprotein bands and analyzed multiclonal paraproteinaemias.
    • Demonstrated applicability to phenotyping other biological fluid proteins.

    Conclusions:

    • This CAM-based immunofixation technique offers a practical and efficient alternative for protein analysis.
    • It is particularly useful for clinical laboratories needing rapid and sensitive paraprotein detection.