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Updated: Aug 8, 2026

Efficient Sporulation of Saccharomyces cerevisiae in a 96 Multiwell Format
Published on: September 17, 2016
Regulation of sterol biosynthesis in Saccharomyces cerevisiae
B Bĕhalová1, M Bláhová, V Bĕhal
1Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague.
Abstract:
Sterol synthesis in Saccharomyces cerevisiae was primarily controlled by the growth rate. At low specific growth rates the intermediates of ergosterol biosynthesis prevailed in cells. At the same time, the total sterol content reached about 6% of dry matter whereas the content of ergosterol was only 2-2.5%, which seems to be the maximum value for S. cerevisiae. After esterification with fatty acids these sterol intermediates are stored in lipid globules together with reserve triacylglycerols. The sporulating S. cerevisiae cells contained 3.5% sterols and 1.5% ergosterol of dry matter.
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