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Related Experiment Videos

A cell cycle checkpoint monitors cell morphogenesis in budding yeast

D J Lew1, S I Reed

  • 1Department of Molecular Cancer Biology, Duke University Medical Center, Durham, North Carolina 27710, USA.

The Journal of Cell Biology
|May 1, 1995
PubMed
Summary
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In budding yeast, inhibiting bud formation triggers a cell cycle delay, preventing errors like binucleate cells. This discovery reveals a novel checkpoint monitoring cell morphogenesis.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Genetics

Background:

  • Cell cycle progression relies on checkpoint controls to ensure faithful completion of prior events.
  • In Saccharomyces cerevisiae, DNA replication and spindle assembly checkpoints prevent nuclear division if these processes fail.

Purpose of the Study:

  • To investigate the role of bud formation in cell cycle regulation.
  • To identify novel checkpoint mechanisms in budding yeast.

Main Methods:

  • Inhibition of bud formation in Saccharomyces cerevisiae.
  • Monitoring nuclear division and cell cycle progression.
  • Analysis of Cdc28 kinase activation and B-type cyclin accumulation.

Main Results:

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  • Inhibition of bud formation caused a significant delay in nuclear division.
  • This delay occurs post-DNA replication and spindle assembly.
  • Delayed activation of Cdc28 kinase, due to tyrosine 19 phosphorylation and reduced Clb1/Clb2 levels, was observed.

    • Conclusions:

    • A novel checkpoint monitoring cell morphogenesis exists in budding yeast.
    • This checkpoint ensures proper cell polarity before nuclear division.
    • It prevents binucleate cells by delaying mitosis when bud formation is disrupted.