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Related Experiment Videos

A novel method for isolating and quantifying urine pathogens collected from gel-based diapers

J Whitehall1, P Shvartzman, M A Miller

  • 1Department of Family Medicine, Sir Mortimer B. Davis-Jewish General Hospital, Montreal, Quebec, Canada.

The Journal of Family Practice
|May 1, 1995
PubMed
Summary

Collecting urine specimens from infants using gel-based diapers is feasible. This method accurately estimates bacterial concentrations in simulated infected urine, offering a reliable diagnostic tool.

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Area of Science:

  • Microbiology
  • Clinical Diagnostics
  • Pediatrics

Background:

  • Children frequently present with wet, gel-based diapers, posing a challenge for obtaining urine specimens.
  • Existing methods for pediatric urine collection can be invasive or difficult to implement.

Purpose of the Study:

  • To develop and validate a method for obtaining reliable urine specimens from gel-based diapers.
  • To assess the accuracy of bacterial concentration estimation from diaper samples.

Main Methods:

  • A blinded clinical laboratory trial was performed.
  • Diaper samples were cut using forceps and scissors, vortexed in saline, and the supernatant was cultured.
  • Simulated infected urines with known concentrations of Escherichia coli were used.

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Main Results:

  • Weight measurements of diaper samples showed reproducible results.
  • Supernatant cultures demonstrated excellent correlation with the initial bacterial inoculum.
  • A 2-hour delay in culturing did not affect the results.

Conclusions:

  • The developed technique accurately and reliably estimates bacterial concentrations in infected urine from gel-based diapers.
  • This method shows promise for clinical implementation in pediatric diagnostics.
  • Further evaluation in clinical settings is recommended.