Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Structural sites specific to citrus viroid groups

M I Francis1, J A Szychowski, J S Semancik

  • 1Department of Plant Pathology, University of California, Riverside 92521, USA.

The Journal of General Virology
|May 1, 1995
PubMed
Summary

This study shows that cDNA probes can specifically detect all citrus viroid group members. Probe specificity is influenced by viroid RNA structure and experimental conditions, suggesting a shared stem-loop conformation.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Draft Genome Sequences of Mycoplasma mycoides subsp. <i>mycoides</i> Strains APF9 and AP108, Isolated in Nigeria in 2014 to 2016.

Microbiology resource announcements·2019
Same author

Finger Imprint of Poncirus trifoliata: A Specific Interaction of a Viroid, a Host, and Irrigation.

Plant disease·2019
Same author

Prevalence and direct economic losses from bovine tuberculosis in makurdi, Nigeria.

Veterinary medicine international·2014
Same author

Correlation of hop stunt viroid variants to cachexia and xyloporosis diseases of citrus.

Phytopathology·2008
Same author

The question of Citrus viroid IV as a cocadviroid.

Archives of virology·2005
Same author

Host-directed processing of Citrus exocortis viroid.

The Journal of general virology·2005

Area of Science:

  • Plant Pathology
  • Molecular Biology
  • Virology

Background:

  • Citrus viroids are small, circular RNA pathogens affecting citrus production worldwide.
  • Accurate detection and differentiation of viroid species are crucial for disease management.
  • Current detection methods may lack specificity across entire viroid groups.

Purpose of the Study:

  • To develop a cDNA probe capable of specifically detecting all members of a citrus viroid group.
  • To investigate factors influencing the specificity of hybridization reactions.
  • To identify structural features responsible for group-specific detection.

Main Methods:

  • Synthesis of cDNA probes using random-priming on a viroid RNA template.
  • Hybridization assays to test probe specificity against various citrus viroid isolates.

Related Experiment Videos

  • Mapping of probe transcription loci on citrus viroid genomes (CEVd, CVd-IIb, CVd-IV).

    • Main Results:

    • Synthesized cDNA probes demonstrated specificity for all tested members within a single citrus viroid group.
    • Hybridization specificity was significantly influenced by viroid RNA template conformation, reverse transcription conditions, and hybridization protocols.
    • Mapping suggested a conserved structural conformation, likely a stem-loop, is responsible for group-specific sequence recognition.

    Conclusions:

    • Random-primed cDNA probes can be designed for broad specificity within citrus viroid groups.
    • Viroid RNA secondary structure plays a critical role in achieving group-specific detection.
    • A proposed stem-loop configuration in the viroid template and target sites explains the observed group specificity.