Related Experiment Videos
[Cytokine mRNA expression in peripheral blood mononuclear cells from hemodialysis and CAPD patients]
1Department of Medicine, Juntendo University School of Medicine, Tokyo, Japan.
Abstract:
Inflammatory cytokines, including TNF-alpha, IL-1 beta and IL-6, have been reported to be responsible for acute or chronic complications and biocompatibility issues in dialysis patients have focused on the role of these cytokines. Expression of mRNA for TNF-alpha, interleukin (IL)-1 beta and IL-6 in peripheral blood mononuclear cells (PBMC) and their plasma concentrations was examined in 10 hemodialysis and 10 continuous ambulatory peritoneal dialysis (CAPD) patients. Plasma TNF-alpha and IL-1 beta levels in hemodialysis, CAPD and in every healthy subject were below the detection limits. Plasma IL-6 concentration was increased significantly in hemodialysis and CAPD patients when compared to normal controls (p < 0.01). Expression of mRNA for TNF-alpha, IL-1 beta and IL-6 in PBMCs was increased significantly (p < 0.01) in hemodialysis and CAPD patients compared to normal subjects. These mRNA expressions were lower in CAPD patients than in hemodialysis patients (p < 0.05). Significant correlation was observed between the plasma IL-6 concentration and PBMC IL-6 mRNA expression in patients on hemodialysis or CAPD (p < 0.01). IL-6 mRNA expression in PBMCs did not correlate with the duration of hemodialysis or CAPD, blood urea nitrogen (BUN) or serum creatinine levels. These findings suggest that mRNA expression indicates stimulation by dialysis procedures in patients, and CAPD is tolerated better than hemodialysis by patients with end-stage renal failure.
Insights
Dialysis patients show increased inflammatory cytokine mRNA in peripheral blood cells. Continuous ambulatory peritoneal dialysis (CAPD) appears better tolerated than hemodialysis, with lower inflammatory responses.
Area of Science:
- Immunology and Nephrology: Focuses on inflammatory cytokine expression in end-stage renal disease patients undergoing dialysis.
Context:
- Dialysis patients often experience complications linked to inflammatory cytokines like TNF-alpha, IL-1 beta, and IL-6.
- Assessing cytokine mRNA expression in peripheral blood mononuclear cells (PBMCs) and plasma levels provides insight into inflammatory responses during dialysis.
Purpose:
- To compare the expression of TNF-alpha, IL-1 beta, and IL-6 mRNA in PBMCs and their plasma concentrations between hemodialysis (HD) and continuous ambulatory peritoneal dialysis (CAPD) patients.
- To investigate the relationship between cytokine mRNA expression, plasma cytokine levels, and clinical parameters in dialysis patients.
Summary:
- Plasma IL-6 levels were significantly elevated in both HD and CAPD patients compared to healthy controls.
- PBMC mRNA expression for TNF-alpha, IL-1 beta, and IL-6 was significantly higher in HD and CAPD patients versus controls, with lower expression observed in CAPD patients.
- A significant correlation was found between plasma IL-6 and PBMC IL-6 mRNA expression in dialysis patients.
Impact:
- Dialysis procedures stimulate inflammatory responses, as indicated by increased cytokine mRNA expression.
- CAPD may be a more biocompatible dialysis modality than hemodialysis for patients with end-stage renal failure, suggesting a potentially better patient tolerance.