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A Large Animal Model for Acute Kidney Injury by Temporary Bilateral Renal Artery Occlusion
Published on: February 2, 2021
Renal reperfusion injury: sequential changes in function and regional albumin extravasation
M Lortie1, B Gauthier, G E Plante
1Department of Pharmacology, University of Sherbrooke, Québec, Canada.
Microvascular Research
|November 1, 1994
Summary
Renal ischemia impairs kidney function and causes albumin-bound Evans blue dye extravasation, particularly in the inner medulla and papilla, after reperfusion. Edema develops later, indicating delayed recovery of kidney health.
Area of Science:
- Nephrology
- Renal Physiology
- Vascular Biology
Background:
- Renal ischemia-reperfusion injury is a significant clinical concern.
- Understanding the functional and structural changes in the kidney post-ischemia is crucial for developing therapeutic strategies.
Purpose of the Study:
- To investigate the effects of acute renal artery occlusion on renal function and albumin-bound Evans blue dye (EB) extravasation in dogs.
- To assess the recovery of renal hemodynamic parameters, filtration, and regional dye distribution following ischemia.
Main Methods:
- Anesthetized dogs underwent 30 minutes of left renal artery occlusion.
- Renal function was assessed by measuring para-amino-hippurate and inulin clearances, urine flow, and sodium excretion.
- Regional EB extravasation and tissue water content were quantified in different kidney regions post-reperfusion.
Main Results:
- Left kidney function was significantly impaired post-reperfusion, with reduced clearances of para-amino-hippurate and inulin.
- Urine flow and sodium excretion were initially decreased but recovered, while hemodynamic parameters showed incomplete recovery.
- Significant EB extravasation occurred in the inner medulla and papilla by 30 minutes of reperfusion, and in the outer medulla by 60 minutes.
- Renal edema became evident only after 60 minutes of reperfusion.
Conclusions:
- Acute renal ischemia leads to significant functional deficits and regional albumin extravasation in the kidney.
- The pattern of EB extravasation suggests specific vulnerability in medullary regions.
- Delayed edema formation indicates a protracted recovery process following renal ischemia-reperfusion.
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