Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Functional interactions between YY1 and adenovirus E1A

J S Lee1, R H See, K M Galvin

  • 1Department of Pathology, Harvard Medical School, Boston, MA 02115, USA.

Nucleic Acids Research
|March 25, 1995
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

p53 expressions: predicting recurrence and second primary tumors in head and neck squamous cell carcinoma.

Journal of the National Cancer Institute·1996
Same author

Randomized trial of epidural versus intravenous analgesia during labor.

Obstetrics and gynecology·1996
Same author

Chronic inhalation of nitric oxide inhibits neointimal formation after balloon-induced arterial injury.

Circulation research·1996
Same author

Evaluation of urine acidification by urine anion gap and urine osmolal gap in chronic metabolic acidosis.

American journal of kidney diseases : the official journal of the National Kidney Foundation·1996
Same author

Sensitivity of the AutoPap 300 QC System for cervical cytologic abnormalities. Biopsy data confirmation.

Acta cytologica·1996
Same author

NeoPath, Inc. NeoPath AutoPap 300 Automatic Pap Screener System.

Acta cytologica·1996

The transcription factor YY1 (Yin Yang 1) represses gene transcription but can be activated by adenovirus E1A. This study identified YY1

Area of Science:

  • Molecular Biology
  • Gene Regulation
  • Virology

Background:

  • YY1 (Yin Yang 1) is a transcription factor belonging to the GLl-Kruppel family.
  • YY1 typically functions as a transcriptional repressor when bound upstream of gene initiation sites.

Purpose of the Study:

  • To map the functional domains of YY1 responsible for repression and activation.
  • To investigate the mechanism by which adenovirus E1A relieves YY1-mediated repression.

Main Methods:

  • Deletion mutagenesis to identify YY1 functional domains.
  • Analysis of protein-protein interactions between YY1 and adenovirus E1A.
  • Construction and characterization of chimeric proteins involving YY1 domains.

Main Results:

Related Experiment Videos

  • The repression domain of YY1 was mapped to its C-terminal region, overlapping the DNA binding domain.
  • An N-terminal activation domain was identified within the first 69 amino acids of YY1.
  • Physical interaction between the YY1 C-terminus and E1A is crucial for E1A-mediated relief of repression and subsequent gene activation.

Conclusions:

  • E1A interaction with YY1's C-terminus is essential for YY1 to respond to E1A.
  • The N-terminal activation domain of YY1, though not directly interacting with E1A, is also necessary for E1A-mediated transcriptional activation.
  • E1A binding likely induces a conformational change in YY1, unmasking the N-terminal activation domain for gene activation.