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Intracellular pH in lizards after hypercapnia

G K Snyder1, J R Nestler, J I Shapiro

  • 1Department of Biology, University of Colorado, Boulder 80309, USA.

The American Journal of Physiology
|April 1, 1995
PubMed
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This study compared how two lizard species, Anolis equestris and Dipsosaurus dorsalis, regulate intracellular pH (pHi) during hypercapnia. Results show species-specific differences in brain and muscle pHi regulation, suggesting active ion transport mechanisms in Anolis equestris.

Area of Science:

  • Comparative Physiology
  • Biochemistry

Background:

  • Intracellular pH (pHi) regulation is crucial for cellular function.
  • Hypercapnia, an increase in CO2 levels, significantly impacts physiological systems.
  • Understanding species-specific responses to hypercapnia is vital for evolutionary and physiological studies.

Purpose of the Study:

  • To investigate the effects of hypercapnia on intracellular pH (pHi) in the brain and skeletal muscle of two lizard species.
  • To compare the pHi regulatory mechanisms between Anolis equestris and Dipsosaurus dorsalis under elevated CO2 conditions.
  • To determine if pHi regulation involves active processes such as transmembrane ion transport.

Main Methods:

  • Utilized the transmembrane distribution of 5,5-[2-14C]dimethyloxazolidine-2,4-dione ([14C]DMO) to measure intracellular pH.

Related Experiment Videos

  • Employed 31P magnetic resonance spectroscopy (NMR) as an independent method for pHi determination.
  • Exposed lizards to controlled hypercapnic conditions (5% CO2) for 60 minutes.

    • Main Results:

    • Brain pHi values determined by DMO and NMR methods were consistent.
    • Dipsosaurus dorsalis exhibited significantly higher resting brain and muscle pHi compared to Anolis equestris.
    • Hypercapnia caused significant decreases in muscle and brain pHi in D. dorsalis, while A. equestris showed a decrease in muscle pHi but no change in brain pHi.

    Conclusions:

    • Intracellular pH regulation during hypercapnia differs significantly between Anolis equestris and Dipsosaurus dorsalis.
    • The smaller change in brain and muscle pHi relative to plasma pH in A. equestris suggests active transmembrane ion transport mechanisms are involved in pHi homeostasis.
    • These findings highlight species-specific adaptations in physiological responses to environmental changes like hypercapnia.