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Related Experiment Videos

Functional genes mapped on the chicken genome

R P Crooijmans1, J J van der Poel, M A Groenen

  • 1Department of Animal Breeding, Wageningen Agricultural University, Wageningen, The Netherlands.

Animal Genetics
|April 1, 1995
PubMed
Summary

Researchers developed novel microsatellite markers from public databases for avian genetics. These markers successfully identified polymorphisms in diverse chicken stocks and reference families, aiding in gene mapping and chromosome assignment.

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Area of Science:

  • Genetics
  • Genomics
  • Animal Breeding

Background:

  • Microsatellite polymorphisms are increasingly utilized in genetic studies.
  • Developing markers from public databases offers an advantage by linking them to known functional genes.

Purpose of the Study:

  • To screen public databases for microsatellite markers and design primers for amplification.
  • To test the utility of these markers in layer and broiler chicken stocks and reference families.
  • To map newly identified microsatellite loci to linkage groups and chromosomes.

Main Methods:

  • Screening of GenBank and EMBL databases for microsatellite sequences.
  • Primer design for amplification of identified microsatellite loci.
  • Testing marker polymorphism on diverse chicken panels and reference families.

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  • Mapping polymorphic loci to established linkage groups and chromosomes.

    • Main Results:

    • 33 microsatellite loci were tested, with 25 showing polymorphism in the test panel.
    • 14 of these polymorphic loci were also polymorphic in reference families.
    • 12 of the 14 mapped loci integrated into previously defined linkage groups.
    • Three loci, previously mapped by in situ hybridization, allowed assignment of linkage groups to specific chromosomes (6, 4, and a microchromosome).

    Conclusions:

    • Public domain databases are a valuable resource for developing functional microsatellite markers in avian species.
    • The developed markers are effective for genetic diversity assessment and linkage mapping in chickens.
    • This study contributes to the chromosomal assignment of linkage groups, advancing avian genome organization understanding.