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Superoxide from glucose oxidase or from nitroblue tetrazolium?
1Department of Biochemistry, Duke University Medical Center, Durham, North Carolina 27710, USA.
Archives of Biochemistry and Biophysics
|April 20, 1995
Abstract:
Glucose oxidase reduces nitroblue tetrazolium, or ferricytochrome c, faster anaerobically than aerobically. This result is inconsistent with the conclusion that GO2 can reduce O2 to O2- which is then responsible for the reduction of NBT and of cytochrome c. Nevertheless, the aerobic reductions are partially inhibitable by superoxide dismutase. A scheme of reactions is proposed which explains why these electron acceptors cause an O2- production which does not occur in their absence when O2 is the sole electron acceptor.