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Ultrasonic methods for sampling human skin micro-organisms

M F Stringer, R R Marples

    The British Journal of Dermatology
    |May 1, 1976
    PubMed
    Summary
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    Ultrasonic skin microflora sampling offers comparable results to manual washing but removes fewer skin cells. This method provides an effective alternative for analyzing skin microbiota.

    Area of Science:

    • Microbiology
    • Dermatology
    • Biophysics

    Background:

    • Human skin harbors a complex microflora crucial for health.
    • Accurate skin microflora sampling is essential for dermatological research and diagnostics.
    • Current standard methods may have limitations in efficiency or cell removal.

    Purpose of the Study:

    • To compare the efficacy of ultrasonic impulse generation for skin microflora sampling against the traditional manual washing method.
    • To evaluate the yield and characteristics of microflora collected by each method.

    Main Methods:

    • Human skin samples were subjected to microflora collection using two techniques: ultrasonic impulse generation and manual rubbing with a washing solution.
    • The ultrasonic method was tested at power settings of 50 W and above.

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  • Collected samples were analyzed for microflora presence and quantity, and corneocyte count.

    • Main Results:

    • The ultrasonic method, at 50 W or higher, demonstrated comparable results to the standard manual washing method in terms of microflora recovery.
    • A key difference observed was that the ultrasonic method removed significantly fewer corneocytes compared to manual washing.
    • This suggests a potentially less invasive sampling approach.

    Conclusions:

    • Ultrasonic impulse generation presents a viable and effective alternative for sampling human skin microflora.
    • The reduced removal of corneocytes by the ultrasonic method may offer advantages in certain research contexts, potentially preserving skin integrity.
    • Further studies could explore optimizing ultrasonic parameters for enhanced microflora capture while minimizing cell disruption.