Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Optimal control of a semibatch fermentation

H Ohno, E Nakanishi, T Takamatsu

    Biotechnology and Bioengineering
    |June 1, 1976
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Effect of loading on the development of nerve fibers around oral implants in the dog mandible.

    Clinical oral implants research·2001
    Same author

    Noninvasive measurement of cerebral blood flow with (99m)Tc-hexamethylpropyleneamine oxime single-photon emission computed tomography and 1-point venous blood sampling.

    Stroke·2000
    Same author

    Indium-111 platelet scintigraphy in a patient with chronic disseminated intravascular coagulation.

    Clinical nuclear medicine·2000
    Same author

    [Progression of silent cerebral infarction in Japanese: a hospital-based study].

    Nihon Ronen Igakkai zasshi. Japanese journal of geriatrics·2000
    Same author

    Solid-phase synthesis of substituted guanidines using a novel acid labile linker.

    Journal of combinatorial chemistry·2000
    Same author

    [B cell acute lymphocytic leukemia with marked leukocytosis and t(3;15)(q27;q2?2)].

    [Rinsho ketsueki] The Japanese journal of clinical hematology·1999
    Same journal

    Growth Model for Continuous Culture of a Hydrogen-Oxidizing Bacterium, Hydrogenophilus thermoluteolus Strain TH-1.

    Biotechnology and bioengineering·2026
    Same journal

    Glycoengineered Recombinant Alpha1-Antitrypsin Results in Comparable In Vitro and In Vivo Activities to Human Plasma-Derived Protein.

    Biotechnology and bioengineering·2026
    Same journal

    Minimizing Off-Target Effects of CRISPR-Cas9 With Optimized sgRNA: Evaluation of Efficiency and Specificity in the Tumor Protein 53 (TP53) Region.

    Biotechnology and bioengineering·2026
    Same journal

    Metabolic Flux Analysis Reveals Cell Line-Specific Rewiring in CHO Cells Following TCA Cycle Intermediate Feeding for Bioprocess pH Control.

    Biotechnology and bioengineering·2026
    Same journal

    Photohydrogenotrophic Cultivation of Purple Non-Sulfur Bacteria in an Open Bioreactor: Enhanced Selectivity Through Light Cycling and Ammonium Limitation.

    Biotechnology and bioengineering·2026
    Same journal

    Translating Blue Light Stimulation From Batch to Perfusion: Process and Intracellular Metabolic Analysis.

    Biotechnology and bioengineering·2026
    See all related articles

    This study optimized amino acid production using semibatch fermentation. Green's theorem determined the optimal substrate feed rate, maximizing lysine production by an auxotrophic mutant.

    Area of Science:

    • Biochemical Engineering
    • Microbial Fermentation
    • Metabolic Engineering

    Background:

    • Semibatch fermentation is a key bioprocess for producing valuable compounds like amino acids.
    • Optimization of fermentation parameters is crucial for maximizing product yield and economic viability.
    • Lysine production using microbial fermentation is significant for animal feed and human nutrition.

    Purpose of the Study:

    • To optimize the semibatch fermentation process for enhanced amino acid production.
    • To determine the optimal substrate feed rate for maximizing the production rate of lysine.
    • To apply Green's theorem as a novel method for fermentation process optimization.

    Main Methods:

    • Utilized a semibatch fermentation system for lysine synthesis.

    Related Experiment Videos

  • Employed an auxotrophic mutant strain for targeted amino acid production.
  • Applied Green's theorem to the mathematical model of the fermentation process to identify optimal conditions.

    • Main Results:

    • Successfully determined the optimal substrate feed rate for the semibatch fermentation.
    • Achieved maximization of the production rate for the desired amino acid (lysine).
    • Demonstrated the efficacy of Green's theorem in optimizing bioprocesses.

    Conclusions:

    • Green's theorem provides an effective mathematical approach for optimizing semibatch fermentation.
    • The identified optimal feed rate significantly enhances lysine production efficiency.
    • This optimization strategy holds potential for improving the industrial production of amino acids.