Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Large-scale oligoribonucleotide production

R H Davis1

  • 1Department of Chemical Engineering, University of Colorado, Boulder 80309-0424, USA.

Current Opinion in Biotechnology
|April 1, 1995
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Initial pastoral visits and communication in depth.

Journal of religion and health·2013
Same author

Discordant identification in critical incident stress.

Journal of religion and health·2013
Same author

Undulant fever.

Kentucky medical journal·2010
Same author

Stokes's cradle: Newton's cradle with liquid coating.

Physical review letters·2010
Same author

Modeling and measurement of yeast flocculation.

Biotechnology progress·2010
Same author

Modeling and optimization of a batch process for in vitro RNA production.

Biotechnology and bioengineering·2008
Same journal

Microbial C1 assimilation pathways for chemical synthesis: from native metabolism to synthetic design.

Current opinion in biotechnology·2026
Same journal

Medicinal plants fermentation: current knowledge and perspectives.

Current opinion in biotechnology·2026
Same journal

Fermented foods: lessons learned from metagenomics.

Current opinion in biotechnology·2026
Same journal

Microfluidic platforms for the transient transfection of mammalian cells: recent developments and challenges.

Current opinion in biotechnology·2026
Same journal

Harvesting insights from recent advances in yeast genomics for predictable and precision wine fermentation.

Current opinion in biotechnology·2026
Same journal

Minimal enzyme cascades for the aromatic-to-aromatic upgrading of lignin monomers.

Current opinion in biotechnology·2026
See all related articles

Producing large quantities of RNA oligonucleotides is crucial for various applications. Research focuses on improving in vitro transcription and chemical synthesis methods for efficient RNA production and stability.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Synthetic Biology

Background:

  • Increasing demand for RNA oligonucleotides in biomedical and agricultural fields.
  • Need for scalable production methods yielding milligram or larger quantities.
  • Existing methods like in vitro transcription and chemical synthesis require optimization.

Purpose of the Study:

  • To review and highlight advancements in RNA oligonucleotide production.
  • To discuss strategies for enhancing efficiency and yield in RNA synthesis.
  • To explore methods for producing stable and active modified RNA analogs.

Main Methods:

  • Optimization of in vitro transcription (IVT) reaction conditions.
  • Development of immobilized templates for IVT.

Related Experiment Videos

  • Advancements in organic chemical synthesis for RNA production.
  • Strategies for creating modified RNA analogs with improved stability.

    • Main Results:

    • Progress in optimizing solution conditions for IVT.
    • Successful implementation of immobilized templates in IVT.
    • Significant improvements in coupling efficiencies for chemical RNA synthesis.
    • Development of modified RNA analogs exhibiting enhanced stability and retained activity.

    Conclusions:

    • Scalable production of RNA oligonucleotides is achievable through optimized IVT and chemical synthesis.
    • Modified RNA analogs offer advantages in stability and functionality for diverse applications.
    • Continued research in synthesis methodologies will further expand RNA applications.